This experimental plan template is an editable Gantt chart for one wet-lab term. It shows two parallel experiments, incubation waits and a fallback if the first experiment fails. All durations are sample figures.
The experiments and durations are sample data for a fictional term, not protocol timings.
The chart covers a sample cell-biology term in six groups:
| Group | Weeks | What happens |
|---|---|---|
| Preparation | 1 to 3 | Agree experiments with the supervisor, order reagents and a backup siRNA, book equipment, expand cells. |
| Experiment A: knockout cell line | 4 to 10 | Deliver Cas9 and guide RNA, check the edited pool, clone single cells, expand and validate them. |
| Experiment B: dose-response assay | 4 to 9 | Three replicates of a routine assay a fortnight apart, then the analysis. |
| Experiment C: knockout phenotype | 11 to 14 | Three replicates comparing knockout and wild-type cells, then the analysis. |
| Fallback: siRNA knockdown | 11 to 13 | Knock the same gene down, confirm it by qPCR and run the phenotype assays on those cells. |
| Analysis and reporting | 11 to 16 | Lab meeting, a buffer week for repeats, figures and the progress report. |
Separate wait bars show elapsed time without bench work. The sample has four: reagent delivery, pool recovery, clone growth and the knockdown taking effect. Schedule the second experiment on the bench days these waits leave free.
Schedule routine assays during the other experiment's waits; reserve a separate day for a new method. The sample puts dose-response replicates inside the knockout's waits and gives Cas9 delivery a day to itself.
Keep each bench day to what fits in a working day; the hour-by-hour steps stay in the protocol.
"Knockout validated: go or switch to the fallback" is a milestone at the end of week 10, and both the phenotype experiment and the siRNA fallback depend on it. Ganttile has no conditional branches, so once you know the result, delete the group you do not need.
The backup siRNA is ordered in week 1, so the fallback never waits on a delivery. If validation runs late, turn on Auto schedule under Settings and lengthen the bar: everything linked after it moves. Use delay tracking steps to follow the changed dates.
Choose the chart for a plan that changes weekly. In a typical spreadsheet plan a slipped date moves nothing after it unless you have built the formulas; here the dependencies and milestones carry it through. The Excel export still gives your supervisor a task table: names, dates and durations, without bars.
Choose Use template to copy the chart and its dependencies into a free account, starting on the coming Monday. For the project around the bench work, use a wider research schedule; for a review of the literature, a review stage schedule.
No. Ganttile's timescale goes down to days, not hours. Plan which day each step happens on and keep the timed steps, such as a 30-minute incubation, in the protocol.
This template covers one term of experiments. Use a whole-degree schedule for the PhD and keep this term plan for bench work.
A free read-only share link lets your supervisor see the current chart without an account. You can also export a PDF for lab meetings.